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KMID : 0903520040470020170
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2004 Volume.47 No. 2 p.170 ~ p.175
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Characterization of Oil-Degradation Biosurfactant Produced by Bacillus sp. TBM40-3
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Kim Sun-Hee
Lee Sang-Cheol
Yoo Ju-Soon
Joo Woo-Hong
Jung Soo-Yeol
Choi Yong-Lark
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Abstract
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In this paper we studied about Bacillus sp. TBM40-3 producing biosurfactants. The strains were isolated from Taeback Mountain soil and identified as Bacillus sp. by 16S rDNA nucleotides sequence analysis. The TBM40- 3 was gram-positive and rod-shaped as observed by field emission scanning microscopy. After the cultivation TBM40-3 in LB broth for 90 h and the surface tension of supernatant was decreased to 29 mN/m. Emulsification activity and stability of crude biosurfactant was measured by using water-immiscible hydrocarbons and oil as substrate. Maximum emulsification activity and stability was obtained from soybean oil. Also, we confirmed that the TBM40-3 producing biosurfactant had an effect on crude oil while showing a superior effect as compared to chemically synthesized surfactants (SDS, Span85, Tween40, Triton X-100). As a result, the Bacillus sp. TBM40-3 producing biosurfactant had potent properties as an emulsifying agent and an emulsion stabilizing agent.
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KEYWORD
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Bacillus sp.TBM40-3, biosurfactant, emulsification activity, emulsification stability
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